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Respiration Vials with Integrated Optical Oxygen and Temperature Sensors

  • Vials with (4 mL or 20 mL) with integrated optical oxygen and temperature sensors 
  • For small scale respirometry or metabolic rate measurements
  • Track oxygen levels simultaneously in the liquid phase and in the gaseous headspace
  • Measures simultaneously temperature and oxygen in gas or water
  • For true temperature compensation of the oxygen measurement
  • Lids with septum
  • No oxygen consumption
  • >3 years shelf time

The new optical temperature sensors from PyroScience are based on an innovative technology utilizing temperature-sensing properties of luminescent indicators. The combined respiration vials have an integrated optical temperature and oxygen sensor for contactless measurements in gaseous or liquid samples. The ready assembled respiration vials with integrated optical temperature and oxygen sensor stripes are available with a volume of ca. 20mL. The separately available adapter rings ADVIAL20T allow for easy fixation of optical fibers connecting the respiration vials to the multi-channel FireSting devices from PyroScience. The elongated shape of the sensors allows simulatenous oxygen and temperature measurements at different heights within the vial by simply sliding the adapter rings up and down. The optical temperature sensor in the combined respiration vial can be used for true temperature compensation of the integrated oxygen sensor for high precision measurements.

Application Examples. True temperature-compensated respiration rate measurements of bacteria or cell cultures, oxygen production/consumption rates of microalgae, general metabolic rate measurements, respiration rate measurements of eggs, larvae or small fish etc.

Typical Applications

Compatible oxygen meters

FireSting pro (FSPRO-2, FSPRO-4)
FireStingO2 (FSO2-2, FSO2-4)

Available Items

Item No. Description
(Volume / Diameter / Height)
Time (s)**
in EU*
TOVIAL20 Vial with integrated optical Oxygen & Temperature sensor Package includes 4 vials with optical isolation
(ca. 20 mL / ca. 28 mm / ca. 61 mm)
<20s (liquid)
<3s (gas)
360 €
ADVIAL20T Adapter ring for 20mL vials with up to 3 sensors 30 €
SEPVIAL20 Lids with Septa (PU=4 pieces)
For replacement in 20mL vials
20 €


Item No. Description Pricing
in EU*
OXCAL Calibrations capsules for 50ml 0% O2 solution, PU=10 capsules, for 0% calibration of optical O2 sensors 44 €
SPFIB-BARE Bare Optical Fiber (1m length), ST-plug + one polished end 50 €
SPFIB-BARE-CL2 Bare Optical Fiber (2m length), ST-plug + one polished end 60 €
ADVIAL20T-KIT Adapter kit including 4x ADVIAL20T and 4x SPFIB-BARE-CL2 290 €

* Only EU-Prices shown. Contact us for pricing outside EU. Not for resale.

** Typical response times for 90% signal change (T90) of oxygen sensor.
     For liquids: measured for the transition from air into a stirred solution of 1% Na2SO3.

Specifications Optical Oxygen Sensor

Given for 2-point calibrated oxygen sensors at 20°C and 1013mbar absolute pressure in default measuring mode.

hPa %O2 %air sat. mg/L
Optimum measuring range 0-500 0-50 0-250 0-23
Maximum measuring range (not specified) 0-1000 0-100 0-500 0-45
Detection limit 0.2 0.02 0.1 0.01
Resolution at 1% O2 0.1 0.01 0.05 0.005
Resolution at 20% O2 0.5 0.05 0.25 0.025
Accuracy at 1% O2 ±0.2 ±0.02 ±0.1 ±0.01
Accuracy at 20% O2 ±2 ±0.2 ±1 ±0.1

Specifications Optical Temperature Sensor

Given for 1-point calibrated flow-through cell with integrated optical temperature sensor at room temperature (20°C).

Measuring range 0 to 50°C (273-323 K; 32-122 °F)
Resolution 0.05°C
Accuracy +/- 0.5°C (1-point calibrated)
Drift <0.5°C / day

General Specifications

Minimum lifetime 10,000,000 data points
Application areas Laboratory, industry, research. Not for medical or any other safety-critical applications. Not for application in humans or in food intended for human consumption.
Compatibility Water/aqueous solutions, gas-phase
Cross sensitivity Other organic solvents, chlorine, bleach
Cleaning 3 % H2O2, ethanol, soap solution
Storage time >3 years in darkness at room temperature
Calibration 1-point for temperature (daily), 1- or 2-point for oxygen
Optical isolation The sensor stripes are covered with a black layer to minimize interference with strong external illumination and light-sensitive / fluorescing samples
Temperature Range 0-50°C (273-323 K; 32-122 °F)