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Sensor Vials with Integrated Optical pH and Temperature Sensors

  • Vials with ca. 20ml and 4ml
  • For small scale metabolic rate measurements
  • Simultaneous pH and T measurements
  • For true temperature compensation of the pH measurement
  • All sensors work with original REDFLASH-technology
  • pH measurements with minimal influence of ionic strength
  • Long-term measurements

 

The new combined vials integrate optical pH and temperature sensors based on an innovative optical detection technology (REDFLASH). The optical temperature sensor can be used for true temperature compensation of the pH sensor for high precision measurements. The pH sensor stripes are fixed at the inner side of the glass and is read-out using optical fibers from the outside. The separately available adapter rings ADVIAL20T allows easy fixation of optical fibres connecting the vial to the new multi-analye FireSting pro optical meter from PyroScience.

The elongated shape of the sensors allows simultaneous measurements at different heights within the vial by simply sliding the adapter rings up and down. The optical temperature sensor can be used for true temperature compensation of the integrated pH sensor for high precision measurements. All sensor stripes are covered with an optical isolation protective layer to prevent interference with the measurement from e.g. external illumination or strong luminescent samples. The optical pH sensors are sensitive to a pH range of 2 pH units. Sensors are offered for two different ranges for pH measurements between pH 6.0 to 8.0 and pH 7.0 to 9.0.

Typical Applications

Small-scale metabolic rate measurements of e.g. cell cultures, eggs, larvae, small crustaceans, small fish, water-, plant-, algal-samples

Compatible oxygen meters

FireSting pro (FSPRO-x)

Available Items

Item No. Description
(Volume / Diameter / Height)
Pricing
in EU*
PHTVIAL20-PK7 Vial with integrated optical pH, oxygen & temperature sensor. pH sensor for measurements between pH 6.0 – 8.0
Package includes 4 vials with optical isolation (ca. 20 mL / ca. 28 mm / ca. 61 mm)
400 €
PHTVIAL20-PK8 Vial with integrated optical pH, oxygen & temperature sensor. pH sensor for measurements between pH 7.0 – 9.0
Package includes 4 vials with optical isolation (ca. 20 mL / ca. 28 mm / ca. 61 mm)
400 €
ADVIAL20T Adapter ring for 20 mL vials 30 €
SEPVIAL20 Lids with Septa (PU=4 pieces)
For replacement in 20mL vials
20 €

Accessories

Item No. Description Pricing
in EU*
PHCAL4 Buffer capsules for 100ml buffer (pH 4.01), PU = 10 capsules, for calibration of optical pH sensors 44 €
PHCAL10 Buffer capsules for 100ml buffer (pH 10.00), PU = 10 capsules, for calibration of optical pH sensors 44 €
SPFIB-BARE Bare Optical Fiber (1m length), ST-plug + one polished end 50 €
SPFIB-BARE-CL2 Bare Optical Fiber (2m length), ST-plug + one polished end 60 €
ADVIAL20T-KIT Adapter kit including 4x ADVIAL20T and 4x SPFIB-BARE-CL2 290 €

* Only EU-Prices shown. Contact us for pricing outside EU. Not for resale.

Specifications Optical pH Sensor

Given for 2-point calibrated pH sensors (pH 4.0 and 10.0) in physiological solutions at 25°C.

PK7 - Version pH
Optimum measuring range 6.0 - 8.0
Resolution at pH 7.0 0.005
Accuracy at pH 7.0 +-0.05
Drift at pH 7.0 <0.005 / day
Response time t90 < 60 sec.
PK8 - Version pH
Optimum measuring range 7.0 - 9.0
Resolution at pH 8.0 0.005
Accuracy at pH 8.0 +-0.05
Drift at pH 8.0 <0.005 / day
Response time t90 < 60 sec.

Specifications Optical Temperature Sensor

Given for 1-point calibrated flow-through cell with integrated optical temperature sensor at room temperature (20°C).



Measuring range 0 to 50°C (273-323 K; 32-122 °F)
Resolution 0.05°C
Accuracy +/- 0.5°C (1-point calibrated)
Drift <0.5°C / day

General Specifications

All versions
Application areas Laboratory, industry, research. Not for medical or any other safety-critical applications. Not for application in humans or in food intended for human consumption.
Temperature range 5 to 50°C
Photostability Negligible photodegradation
Interferences Chlorine, bleach, surfactant solutions, organic solvents. Commercial buffer solutions (typically contain stabilizer) must not be used
Salinity Compensation of ionic strength (IS) for different application areas (10 mM – 500mM IS) possible
Background fluorescence Minimized due to REDFLASH technology
Cleaning Rinsing with deion. water only
Storage time Original packaging (unpacked) 12 months at room temperature
Storage conditions Dark and dry conditions
Calibration 2-point calibration, pH offset adjustment possible
Calibration solution (pH) PyroScience buffer capsules or a self-made buffer must be used. Important: other pH buffers must not be used and can damage the sensor irreversibly
Optical isolation The sensor is equipped with an optical isolation to minimize interference from strong external illumination and light-sensitive / fluorescent samples