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Microprofiling

Microsensors are an important tool in an ever-growing diversity of research fields. Because microsensors need precise positioning down to ca. 10 µm spatial resolution, microprofiling setups became the standard choice for many microsensor applications. Such setups have been successfully applied in:

  • biogeochemistry (e.g. marine and freshwater sediments)
  • biofilms (e.g. marine phototrophic biofilms)
  • microbial mats (e.g. hypersaline cyanobacterial mats)
  • microbial communities in extreme environments (hot springs)
  • insect physiology (e.g. termite gut physiology)
  • plant physiology (e.g. oxygen transport in eelgrass roots)

PyroScience offers microprofiling setups for varying scientific demands. Basic setups are manually operated consisting of e.g. Heavy Stand HS1, Micromanipulator MM33, and the FireSting. The advanced setups are controlled by the microprofiling software Profix running on a Windows PC, which operates a motorized micromanipulator (e.g. MU1 with motorized z-axis, MUX2 with motorized z- and x-axis) and reads in data from up to two microsensor modules. Such systems allow complex automized microprofiling applications (e.g. microprofiling at defined time intervals, or automatic transects). Other advanced features are: automatic µM calculation for oxygen microsensors, interactive flux calculations on measured profiles, input file generation for the microprofile analysis program PROFILE from Peter Berg.

 

Possible Setup Configurations

The simplest and most economic setup is based on the manual micromanipulator MM33, which is generally sufficiant for measuring a few microprofiles by moving a micrometer screw stepwise manually. If you need to measure many microprofiles, you should consider the automized setups based on the motorized micromanipulators MU1 or MUX2 in combination with the control software Profix for Windows 7/8/10.

 

Applicable Oxygen Sensor Types

 

Related Peer-Reviewed Publications

Oxygen-dependent niche formation of a pyrite-dependent acidophilic consortium built by archaea and bacteria
Ziegler et al., 2013, The ISME Journal
https://doi.org/10.1038/ismej.2013.64

Compartmentalized microbial composition, oxygen gradients and nitrogen fixation in the gut of Odontotaenius disjunctus
Ceja-Navarro et al., 2014, ISME Journal
https://doi.org/10.1038/ismej.2013.134

Radiative energy budget reveals high photosynthetic efficiency in symbiont-bearing corals
Brodersen et al., 2014, Journal of the Royal Society Interface
https://doi.org/10.1098/rsif.2013.0997

Microbial Iron Oxidation in the Arctic Tundra and Its Implications for Biogeochemical Cycling
Emerson et al., 2015, Applied and Environmental Microbiology
http://doi.org/10.1128/AEM.02832-15

Evidence for water-mediated mechanisms in coral–algal interactions
Jorissen et al., 2016, Proceedings of the Royal Society B
http://doi.org/10.1098/rspb.2016.1137

Regulation of Intertidal Microphytobenthos Photosynthesis Over a Diel Emersion Period Is Strongly Affected by Diatom Migration Patterns
Cartaxana et al., 2016, Frontiers in Microbiology
http://doi.org/10.3389/fmicb.2016.00872

Pseudomonas aeruginosa Aggregate Formation in an Alginate Bead Model System Exhibits In Vivo-Like Characteristics
Sønderholm et al., 2017, Applied and Environmental Microbiology
https://doi.org/10.1128/AEM.00113-17