Pyro Workbench

Luminescence Background Compensation

A background compensation is recommended for optical fibers used for read-out of contactless sensors and for robust probes. For robust probes, respiration vials, flow-through cells and sensor spots with a black optical fiber (sensor type: S, W, T, P, X, U), the FIBER LENGTH needs to be entered into the software for an automatic background compensation (recommended for most applications).

For precision applications, for applications with low signal intensities and for application of nanoprobes, the option MANUAL background compensation must be used.

Fiber Length

Based on the Fiber Length (m) entered in the Settings, a background signal for compensation is estimated automatically by the Pyro Workbench. An averaged and typical luminescence background of the applied black 1 mm optical fiber will be considered.

Manual

For precision applications, for measurements at low signal intensities and for application of oxygen nanoprobes in microfluidic applications, a Manual background compensation must be performed to determine the individual luminescence background of the applied optical fibre. Especially in the case of oxygen nanoparticles (item no. OXNANO) the luminescence background compensation is important. Please ensure that during manual background compensation the Optical Fiber is connected to the medium WITHOUT oxygen nanoprobes.

For other contactless sensors, it is important that the fiber is not attached to the sensor spot (i.e. disconnect this end from the adapter, adapter ring or from the flow-through cell). Please ensure that during the subsequent calibration process the Optical Fiber is again attached to the medium WITH oxygen nanoprobes or to the position with sensor spots. Remind that the position of the spot adapter or adapter ring should not be changed after calibration of the sensor spot; otherwise it has to be calibrated again.