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Pyro Workbench

Manual Luminescence Background Compensation

The calibration of contactless sensors (i.e. sensor spots, flow-through cells, respiration vials and oxygen nanoprobes) includes a compensation of potential background fluorescence from the fiber-optic cable connecting the device with the contactless oxygen sensor. Based on the Fiber Length (m) entered in the Settings, a background signal for compensation is estimated automatically by the Pyro Workbench. The user usually does not notice the background compensation at all. For standard applications this should be the preferred procedure.

But for precision applications and especially for low signal intensities or for applications utilizing oxygen nanoprobes (item no.: OXNANO), a manual background compensation should be performed by the user.

For sensor spots, flow-through cells and respiration vials, please select Custom (advanced) for the upper calibration point and select Manual when asked later for the luminescence background compensation method.

In the case of oxygen nanoprobes this selection possibility is additionally included for the calibration in air saturated water (upper calibration point).

  • After selecting Manual and clicking Next, a manual background compensation can be performed:

Here, the background fluorescence of the connected optical fiber or for oxygen nanoprobes, of the medium   used for the measurements, can be compensated. For this it is important that:

  • one end of the optical fiber is connected to the corresponding channel of the device and
  • the other end of the optical fiber is not attached to the sensor spot, flow-through cell, respiration vial or oxygen nanoprobes (i.e. disconnect this end from the spot adapter, adapter ring or from the flow-through cell)

For the manual luminescence background compensation, wait for steady-state and press the button Take value.

Alternatively, click Next without taking a new value. Then the last values for the background compensation are kept.

After that, the program proceeds with the main calibration window. It is important that for the subsequent calibration process the optical fiber is again attached to the sensor spot, flow-through cell, respiration vial or oxygen nanoprobes, e.g. by connecting this optical fiber end again to the spot adapter, adapter ring or to the flow-through cell.

Please ensure that during background compensation the optical fiber is not connected to the contactless sensor.

Please ensure that during the subsequent calibration process the optical fiber is again attached to the contactless sensor.

Remind that the position of the spot adapter or adapter ring must  not be changed after calibration of the sensor spot; otherwise it has to be calibrated again.