Home  - Privacy Policy  - Legal Imprint
products
 

Respiration Vials with Integrated Optical Oxygen, pH, and Temperature Sensors

  • Vials with ca. 20 ml 
  • Simultaneous pH, O2 and T measurements
  • For small scale metabolic rate measurements
  • All sensors work with original REDFLASH-technology
  • For true temperature compensation of the oxygen and pH measurement
  • Track oxygen levels in the liquid phase and simultaneously in the gaseous headspace
  • No oxygen consumption
  • pH measurements with minimal influence of ionic strength
  • Lids with septum

The new combined respiration vials integrate optical pH, oxygen and temperature sensors based on an innovative optical detection technology (REDFLASH). The ready assembled vials with integrated optical pH, oxygen and temperature sensor stripes enable multi-analyte contactless measurements in liquid or gaseous samples. Separately available adapter ring ADVIAL20T allow for easy fixation of optical fibres connecting the respiration vial to the new multi-analyte FireSting pro optical meter from PyroScience.

The elongated shape of the sensors allows simultaneous measurements at different heights within the vial by simply sliding the adapter rings up and down. The optical temperature sensor can be used for true temperature compensation of the integrated oxygen and pH sensors for high precision measurements. All sensor stripes are covered with an optical isolation protective layer to prevent interference with the measurement from e.g. external illumination or strong luminescent samples. The optical pH sensors are sensitive to a pH range of 2-3 pH units. Sensors are offered for two different ranges for pH measurements between pH 6.0 to 8.0 and pH 7.0 to 9.0.

Typical Applications

  • Small-scale respirometry and metabolic rate measurements of e.g. cell cultures, eggs, larvae, small crustaceans, small fish, water-, plant-, algal-samples etc.
  • Simultaneous oxygen measurements in a liquid phase and a gaseous headspace within the same vial (using 2 adapter rings)

Compatible oxygen meters

FireSting pro (FSPRO-x)

Available Items

Item No. Description
(Volume / Diameter / Height)
Pricing
in EU*
PHTOVIAL20-PK7 Vial with integrated optical pH, oxygen & temperature sensor. pH sensor for measurements between pH 6.0 – 8.0
Package includes 2 vials with optical isolation (ca. 20 mL / ca. 28 mm / ca. 61 mm)
290 €
PHTOVIAL20-PK8 Vial with integrated optical pH, oxygen & temperature sensor. pH sensor for measurements between pH 7.0 – 9.0
Package includes 2 vials with optical isolation (ca. 20 mL / ca. 28 mm / ca. 61 mm)
290 €
ADVIAL20T Adapter ring for 20 mL vials 30 €
SEPVIAL20 Lids with Septa (PU=4 pieces)
For replacement in 20mL vials
20 €

Accessories for Connection to Optical Meters

Item No. Description Pricing
in EU*
SPFIB-BARE Bare Optical Fiber (1m length), ST-plug + one polished end 50 €
SPFIB-BARE-CL2 Bare Optical Fiber (2m length), ST-plug + one polished end 60 €
ADVIAL20-KIT Adapter kit including 4x ADVIAL20 and 4x SPFIB-BARE-CL2 290 €

* Only EU-Prices shown. Contact us for pricing outside EU. Not for resale.

Specifications Optical Oxygen Sensor

Given for 2-point calibrated oxygen sensors at 20°C and 1013mbar absolute pressure in default measuring mode.


hPa %O2 %air sat. mg/L
Optimum measuring range 0-500 0-50 0-250 0-23
Maximum measuring range (not specified) 0-1000 0-100 0-500 0-45
Detection limit 0.2 0.02 0.1 0.01
Resolution at 1% O2 0.1 0.01 0.05 0.005
Resolution at 20% O2 0.5 0.05 0.25 0.025
Accuracy at 1% O2 ±0.2 ±0.02 ±0.1 ±0.01
Accuracy at 20% O2 ±2 ±0.2 ±1 ±0.1

Specifications Optical pH Sensor

Given for 2-point calibrated pH sensors (pH 4.0 and 10.0) in physiological solutions at 25°C.

PK7 - Version pH
Optimum measuring range 6.0 - 8.0
Resolution at pH 7.0 0.005
Accuracy at pH 7.0 +-0.05
Drift at pH 7.0 <0.005 / day
Response time t90 < 60 sec.
PK8 - Version pH
Optimum measuring range 7.0 - 9.0
Resolution at pH 8.0 0.005
Accuracy at pH 8.0 +-0.05
Drift at pH 8.0 <0.005 / day
Response time t90 < 60 sec.

Specifications Optical Temperature Sensor

Given for 1-point calibrated flow-through cell with integrated optical temperature sensor at room temperature (20°C).



Measuring range 0 to 50°C (273-323 K; 32-122 °F)
Resolution 0.05°C
Accuracy +/- 0.5°C (1-point calibrated)
Drift <0.5°C / day

General Specifications

All versions
Application areas Laboratory, industry, research. Not for medical or any other safety-critical applications. Not for application in humans or in food intended for human consumption.
Temperature range 5 to 50°C
Photostability Negligible photodegradation
Interferences Chlorine, bleach, surfactant solutions, organic solvents. Commercial buffer solutions (typically contain stabilizer) must not be used
Salinity Compensation of ionic strength (IS) for different application areas (10 mM – 500mM IS) possible
Background fluorescence Minimized due to REDFLASH technology
Cleaning Rinsing with deion. water only
Storage time Original packaging (unpacked) 12 months at room temperature
Storage conditions Dark and dry conditions
Calibration 2-point calibration, pH offset adjustment possible
Calibration solution (pH) PyroScience buffer capsules or a self-made buffer must be used. Important: other pH buffers must not be used and can damage the sensor irreversibly
Optical isolation The sensor is equipped with an optical isolation to minimize interference from strong external illumination and light-sensitive / fluorescent samples